Core Facility Gene Editing

The Core Facility Gene-Editing offers you a full-service for the generation of new genetically modified mouse lines at the SPF mouse facility of LIMES-GRC. In addition, we perform all transgenic standard services like rederivation of mouse lines, IVF, cryopreservation or microinjection into oocytes and blastocysts.

To establish a new mouse model, we arrange a first meeting where we can discuss your project ideas and figure out the method of choice for your needs. Of course, advice is provided free of charge. The following Gene-Editing workflow is described in more detail here.

Depending on the complexity of the project and different material input we apply a staggered price chart for the CRISPR/Cas9-mediated generation of gene-edited mice. Prices apply for internal users. However, we also welcome external users in our Core Facility.

Our Core Facility operates a state-of-the-art injection microscope and electroporation set-ups for manipulation of oocytes and 2-cell embryos.
Resulting mice are born and kept in our SPF animal facility with very strict hygiene standards.